Thermal detoxification of the venom from Daboia russelli russelli of Eastern India with restoration of fibrinolytic activity.

摘要: The fibrinolytic components of venom have been evaluated for long in the enzymatic treatment thrombosis. Russell’s viper has activity that is associated with hemorrhagic activity. Here it investigated whether crude could be detoxified by thermal denaturation retaining at 0.05 mg/ml 20 mM K-phosphate, pH 7.5 when exposed to 100 °C 5 min followed cooling 25 °C 1 h led its detoxification, while 80–85% was recovered. Assessment toxicity renatured mice after injection 5 fold excess lethal dose showed no lethality including hemorrhage, myotoxicity, cytotoxicity and liver toxicity. This simple method preparation component therapeutic use may developed.