Purification and characterization of a low molecular weight multifunctional cytotoxic phospholipase A2 from Russell's viper venom.
作者： G MAITY , S MANDAL , A CHATTERJEE , D BHATTACHARYYA
DOI: 10.1016/J.JCHROMB.2006.08.038
关键词:
摘要: A basic toxin from Russell's viper venom of 7.2 kDa (RVV-7) has been purified to homogeneity after partial unfolding by 4 M urea followed filtration through Centricon-30 membrane. Its N-terminal sequence showed strong homology with snake cytotoxins. Cytotoxic activity RVV-7 demonstrated B16F10 melanoma cells. PLA2 was observed in cytotoxin (CX3) Naja kauthia bearing RVV-7. Phospholipase A2 and trypsin inhibitory activities were also Chemical modification inhibition studies suggested independent functional sites for these activities. qualitative assessment tumor growth made.
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