Modification of histidyl residues in proteins by diethylpyrocarbonate.

摘要: Publisher Summary Diethylpyrocarbonate has been shown to react specifically or stoichiometrically with a single histidyl residue in certain proteins. In other cases, the modification of activity correlated one more residues despite possible residues; this correlation is facilitated by fact that hydroxylamine removes carbethoxy group from modified and tyrosyl residues, but not lysyl sulfhydryl residues. Several enzymes have be inactivated than residue. Thus, although diethylpyrocarbonate does always proteins, it selective acylating agents can give useful information about role many This chapter describes way optimal conditions for reaction should determined side examined.