Diethyl pyrocarbonate: An examination of its properties in buffered solutions with a new assay technique
作者： Shelby L. Berger
DOI: 10.1016/0003-2697(75)90315-2
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摘要: Abstract Two assay systems for diethyl pyrocarbonate are described. The first is a spectrophotometric method that makes use of the rapid reaction with colored compound, 5-thio-2-nitrobenzoate, to form colorless product. second based on inactivation lactate dehydrogenase and, unlike first, can be used in presence thiols. rate decomposition has been studied several buffers at different pH values.
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