Characterization of the aqueous extract of the root of Aristolochia indica: evaluation of its traditional use as an antidote for snake bites

摘要: Abstract Ethnopharmacological relevance The aqueous extract of the roots Aristolochia indica is used as a decoction for ailment number diseases including snake bite treatment. Though alcoholic different parts plant are well studied, information on limited. We have estimated aristolochic acid, enzymes, enzyme inhibitors and anti-snake venom potency its root extract. Materials methods Reverse phase–HPLC was to quantify acid. Zymography, DQ-gelatin assay atomic force microscopy were done demonstrate gelatinase collagenase activities SDS-PAGE followed by MS/MS analysis revealed identity major protein components. Toxicity animal model. Interaction with Russell's viper components Rayleigh scattering assay. Results acid content 3.08±1.88×10−3 mg/ml. possesses strong gelatinolytic, collagenase, peroxidase nuclease together l -amino oxidase protease inhibitory potencies. Partial proteomic studies indicated presence starch branching enzymes constituent did not show any acute sub-chronic toxicity in animals at lower doses, but high dose causes liver kidney damage. elongated duration survival after application venom. Conclusions Considering low extract, consumption short time moderate does appear cause serious toxicity. Strong inhibition may give partial relief from topical