AKT activation drives the nuclear localization of CSE1L and a pro-oncogenic transcriptional activation in ovarian cancer cells.

摘要: The human homolog of the yeast cse1 gene (CSE1L) is over-expressed in ovarian cancer. CSE1L forms complex with Ran and importin-α has roles nucleocytoplasmic traffic expression. accumulated nucleus cancer cell lines, while it was localized also cytoplasm other lines. Nuclear localization depended on AKT, which constitutively active cells, as protein translocated to when AKT inactivated. Moreover, expression a forced translocation from cells. accrual associated nuclear accumulation phosphorylated Binding 3 (RanBP3), well. Also samples cancer, activation phosphorylation RanBP3. Expression profiling cells after silencing showed that required for genes promoting invasion metastasis. In agreement, impaired motility invasiveness Altogether these data show activated by affecting RanBP3 determines likely concentration transcription factors conveying pro-oncogenic signals.