An element downstream of the cap site is required for transcription of the gene encoding mouse ribosomal protein L32.

摘要: Abstract To identify the elements that regulate transcription of mouse gene encoding ribosomal protein L32 (rpL32), we transfected monkey kidney (COS or CV-1) cells with mutants bearing progressive 5' deletions an internal deletion in exon I and measured their transient expression by S1 nuclease protection analysis. When mutant genes were tested vector pi SVHSplac, which contains a short segment oriregion simian virus 40, maximum was observed as little 36 base pairs flanking sequence, expressed very efficiently. However, when simple prokaryotic (pUC) vector, increased 3- to 4-fold sequences between -36 -159, absolutely required for expression. Gel mobility-shift methylation interference analyses revealed nuclear factor specifically binds GGCTGCCATC sequence within this segment. These results, taken together other recent findings, indicate involved transcriptional regulation rpL32 are distributed over 200-base-pair region spans cap site. The contributions some these apparently masked presence 40 ori-region elements.