The mouse ribosomal protein L7 gene. Its primary structure and functional analysis of the promoter region.

摘要: The expressed gene coding for mouse ribosomal protein L7 (rpL7) was structurally and functionally characterized. It consists of seven exons, spans 3107 base pairs, its sequence initiates within exon 1. primary structure rpL7 (270 amino acids), as inferred from the nucleotide exons gene, cDNA, is 12 residues longer than rat counterpart. shares common structural features with most other mammalian genes analyzed thus far. These include lack a canonical TATA box major transcription initiation site at cytidine residue embedded in stretch 14 pyrimidines, flanked by C + G-rich regions. Transient expression assays revealed that promoter region bears several regulatory elements, both upstream to capsite transcribed portion gene. One internal element assigned first intron second one 20-base pair spanning exon-intron junction. activity deletion mutant rpL32 lacking elements can be rescued insertion, sense orientation, corresponding unique spatial organization well murine examined far, might indicate this architecture involved mechanism coordinating their expression.