Inactivation of the Transcriptional Regulator-Encoding Gene sdiA Enhances Rice Root Colonization and Biofilm Formation in Enterobacter cloacae GS1
作者： M. Shankar , P. Ponraj , D. Illakkiam , J. Rajendhran , P. Gunasekaran
DOI: 10.1128/JB.01236-12
关键词: Microbiology 、 Mutant 、 Rhizosphere 、 Homoserine 、 Enterobacter cloacae 、 Colonization 、 Bacteria 、 Biology 、 Biofilm 、 Cell adhesion
摘要: Enterobacter cloacae GS1 is a plant growth-promoting bacterium which colonizes rice roots. In the rhizosphere environment, N-acyl homoserine lactone (NAHL)-like quorum-sensing signals are known to be produced by host plants and other microbial inhabitants. E. was unable synthesize NAHL but had NAHL-dependent transcriptional regulator-encoding gene sdiA. This study aimed at understanding effects of SdiA cross talk in root colonization GS1. Pleiotropic sdiA inactivation included substantial increases biofilm formation, suggesting negative role for bacterial adhesion. We provide evidence that leads elevated levels biosynthesis curli, involved cellular Extraneous addition NAHLs effect on formation. However, mutant insensitive NAHLs, this NAHL-induced inhibition formation dependent. Therefore, it proposed both microbes act as cross-kingdom interspecies negatively impact adhesion and, thereby,
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