Radial keratotomy: I. The wound healing process and measurement of incisional gape in two animal models using in vivo confocal microscopy

摘要: Using in vivo confocal microscopy, corneal wound healing was evaluated both rabbit and cat eyes after radial keratotomy. A total of six were sequentially over time for 1 mo surgery by quantitative measurements changes incisional gape determined. In histopathologic correlated with conventional findings 18 4 eyes; the animals killed at various intervals from 0-30 days surgery. rabbit, characterized initial ingrowth epithelium followed persistence within without a marked fibrotic response. Measurement showed increasing 144 +/- 32 microns on day 0 to 976 155 26 distance 2.4 mm optical zone. These not significantly different (P = 0.996) those obtained using techniques which an 252 112 917 216 2.5 eyes, keratotomy wounds increase 135 56 245 88 7 Starting 14 continuing 30, there progressive decrease 198 41 92 35 microns. Sequential, analyses indicated that retention wound. Initiation decreasing associated replacement epithelial plug fibroblastic tissue. observed suggest involves contraction response cells. Furthermore, contractile appears be biphasic involving precontractile phase. Overall these data microscopy provides living tissue comparable dead, fixed, sectioned Additionally, absence fibrosis model raises important questions as appropriateness this experimental human