Isolation and characterization of 6-hydroxymethylpterin as a Crithidia growth-promoting factor from spinach chloroplasts.

摘要: Publisher Summary This chapter discusses the characterization of 6-hydroxymethylpterin as a Crithidia growth-promoting factor from spinach chloroplasts. The has been isolated chloroplasts by chromatography on DEAE-Sephadex and cellulose columns that is usual method to separate natural folate compounds. Phenolic compounds in whole leaves derivatives have found present at 100-fold greater concentration than pteridine compounds, but similar fluorescence maxima, sometimes interfere with fluorimetric used identify leaves. In contrast, 2-amino-4-hydroxy-6-alkylpteridines (6-alkylpterins) promote growth trypanosomid flagellate fasciculata when grown chemically defined medium limited folic acid. response 6-alkylpterins about 1000 times lower derivatives. Thus, method, using excitation 360 nm emission 440 nm, biological assay procedure, C. test organism, are quantify 6-alkylpterin during their isolation To pterins, gas liquid chromatographymass spectrometry used. describes detail Elution profile Sephadex G-25 column also discussed this chapter.