Stabilizing Action of Tetrahydrobiopterin on the Activity of Neuronal Nitric Oxide Synthase
作者： V. N. Tsyvkin
DOI: 10.1007/S11062-012-9305-X
关键词: Cerebellum 、 Biophysics 、 Enzyme 、 No synthase 、 Neuronal Nitric Oxide Synthase 、 Chemistry 、 NOS1 、 Tetrahydrobiopterin 、 Cytoplasm 、 Arginine
摘要: We studied the effect of tetrahydrobiopterin (BH4), one co-factors NO synthase (NOS), on process inactivation this enzyme in a cytoplasmic fraction obtained from rat cerebellar tissue. Tetrahydrobiopterin stimulated dose-dependent manner activity NOS measured according to accumulation [3H]-citrulline. In course reaction at 37°С without addition BH4, enzymatic rapidly decreased; presence 10 μM rate was noticeably smaller. Preincubation (40 min long) under same conditions but absence substrates (arginine and NADPH) BH4 also led decrease activity. 1 mM not only slowed down provided an appreciable increase its activity, as compared with variant preincubation. This fact is indicative BH4-free inactive form above-mentioned fraction, which can be additionally activated. The data show that stabilizes due occurring independently realization reaction.
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