Molecular Characterization of a Hamster Oviduct-Specific Glycoprotein

摘要: There is growing evidence that the oviduct not a passive conduit for gamete and embryo transport but serves function gametes and/or embryos. The oviductal epithelium secretes one or more specific glycoproteins associate with egg after ovulation. Several published reports including our preliminary studies have suggested egg-associating glycoprotein(s) from exists in several mammalian species golden hamster. However, little almost no biochemical characterization of hamster oviduct-specific glycoprotein (HOGP) has been reported. To analyze molecular structure HOGP detail, we attempted cloning cDNA corresponding to HOGP. A library constructed phage vector lambda ZAPII was screened digoxigenin-labeled, baboon as probe. single positive clone isolated, nucleotide sequence isolated determined. Rapid amplification end carried out obtain proximal 5' clone. consisted 2387 bp, coding region contained 2013 bp translating 671 amino acids. acid deduced confirmed chemically determined NH 2-terminal derived signal peptide (21 acids) 650 acids (70 890 daltons) mature form region. appeared eight potential N-glycosylation sites. Northern blot analysis revealed message approximately 2.5 kb present RNA other tissues. showed high homology baboon, bovine, human glycoprotein. These results demonstrate an homologue gene various rodent.