tRNA Processing Nucleases

摘要: This chapter focuses on ribonucleases that are involved in generating the 3' terminus of tRNA precursors and cleaving a from multicomponent transcript. Much our knowledge about tRNA-processing pathways has come studies with Escherichia coli bacteriophage-infected E. coli, although there been some examination other bacteria as well. The major interest is due primarily to availability, this system, mutations interfere maturation, allowing processing intermediates be identified nucleases implicated pathway. However, isolated these mutant strains have undergone partial processing. First, portions polycistronic cleaved RNAs which they co-transcribed. Second, multimeric generally converted monomeric or dimeric forms. A goal study any metabolic pathway identify enzymes catalyze each its reactions, understand their specificity mechanism action, determine whether subject regulation, either directly activity synthesis. presents summary exoribonucleases endoribonucleases Given availability lacking many enzymes, alone combination, it likely details will forthcoming near future.