Crude extract of Trichoderma elicits agarwood substances in cell suspensionculture of the tropical tree, Aquilaria malaccensis Lam.
作者： Shashita JAYARAMAN , Rozi MOHAMED
DOI: 10.3906/TAR-1404-63
关键词: Callus 、 Agarwood 、 Aquilaria malaccensis 、 Botany 、 Thymelaeaceae 、 Mycelium 、 Biology 、 Trichoderma 、 Aquilaria 、 Cell culture
摘要: Agarwood is the precious fragrant wood produced by tropical tree Aquilaria, often after elicitation wounding or fungal attack. In this study we established a cell suspension culture of A. malaccensis from leaf-derived callus and induced agarwood production in using elicitors. Elicitors were made crude mycelial extracts two species genera Trichoderma Lasidiplodia. The elicitors added to culture, initiated with 2 g fresh calli, at concentrations ranging 10 mg L-1. A light scent was detected elicited 8 L-1 extract. To increase intensity, cultures calli treated combination inoculum extract most intense fragrance, one comparable scent. harvested, extracted methanol, analyzed GC-MS. Several important compounds including 8-epi-.gama.-eudesmol, Ai-guaiene, alloaromadendrene oxide-1. appeared be suitable inducer for when used an optimal concentration Aquilaria.
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