Gene expression analysis associated with agarwood formation in Aquilaria malaccensis

摘要: Aquilaria malaccensis produces exorbitant aromatic wood known as agarwood. The fragrant is primarily utilized for its aroma a retainer of perfume and medicinal values, making it high-value product suitably called liquid gold. Only infected trees produce agarwood which are exploited oil extraction resulting in indiscriminate cutting inclusion Appendix II the CITES. current research aims to understand changes gene expression during deposition A. under natural condition. Expression 25 genes involved terpenoids biosynthesis was assessed by semi-quantitative PCR followed qRT-PCR using RNA obtained from non-infected wood. Seven housekeeping were also screened which, GAPDH served best potential internal reference gene. Our results showed that, nine primer pairs tested can differentiate healthy sesquiterpenoid pathway up regulated 41.59 fold compared suggests involvement these formation. Sesquiterpene found be express more naturally plants artificially induced plants.