Sequencing of the Canine Cytochrome P450 CYP2C41 Gene and Genotyping of Its Polymorphic Occurrence in 36 Dog Breeds.

摘要: Cytochrome P450 (CYP) drug metabolizing enzymes play an important role in efficient metabolism and elimination. Many CYPs are polymorphic and, thereby, can vary between individuals. In the case of canine CYP2C41, gene polymorphism was identified. However, as first available genome sequences all were CYP2C41 negative, this could not be clarified at genomic level. The present study provides exact characterization deletion level presents a PCR-based genotyping method that used for 1,089 individual subjects from 36 different dog breeds. None Bearded Collie, Bernese Mountain, Boxer, Briard, French Bulldog or Irish Wolfhound had their genomes. contrast, Chinese Char-Pei, Siberian Husky, Schapendoes Kangal breeds, allele frequency very high, with values 67, 57, 43, 34%, respectively. Interestingly, site identical negative dogs, positive dogs showed highly homologous sequence domains upstream downstream gene. now routinely future pharmacokinetic studies canines, order to identify genetically-based poor extensive metabolizers. This, together more vitro screening substrates will help determine clinical relevance optimize treatment. Although relative abundance protein liver seems CYP substantially contribute hepatic expressing both alleles when shows higher catalytic activity given than other metabolic enzymes.