Isolation of a New Canine Cytochrome P450 cDNA from the Cytochrome P450 2C Subfamily (CYP2C41) and Evidence for Polymorphic Differences in Its Expression
作者： Joyce A. Goldstein , Joyce Blaisdell , Stephen A. Bai
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摘要: Two members of the canine cytochrome P4502C subfamily [CYP2C21 and CYP2C41 (sequence has been submitted to Genbank with accession number AF016248)] were cloned from three beagle liver cDNA libraries. The two CYP2C cDNAs exhibited 70% nucleotide amino acid identity as well 74-83% 67-76% human CYP2Cs. Canine is more homologous CYP2Cs than CYP2C21. a slightly lower (66-77%) rat P450CYPs, 2C11 2C12. Reverse transcription-polymerase chain reaction-based restriction enzyme tests for CYP2C21 2C41 mRNAs polymerase genomic DNA developed. was present in livers all dogs tested (N = 9), but only 1 9 (11%). Genomic found that gene coding also 25), which 15 beagles 10 mixed breeds. In contrast, 16% (4 out 25) dogs. An even distribution between sexes This unique polymorphism may be source variability metabolic clearance xenobiotics are metabolized by P450 2C enzymes.
参考文章(26)
H Yoshioka, K Morohashi, K Sogawa, T Miyata, K Kawajiri, T Hirose, S Inayama, Y Fujii-Kuriyama, T Omura, Structural analysis and specific expression of microsomal cytochrome P-450(M-1) mRNA in male rat livers. Journal of Biological Chemistry. ,vol. 262, pp. 1706- 1711 ,(1987) , 10.1016/S0021-9258(19)75695-6
Komori Masayuki, Shimada Hitoshi, Miura Toshiaki, Kamataki Tetsuya, Interspecies homology of liver microsomal cytochrome P-450 Biochemical Pharmacology. ,vol. 38, pp. 235- 240 ,(1989) , 10.1016/0006-2952(89)90032-4
U A Meyer, M Blum, R Gaedigk, A Gaedigk, M Eichelbaum, Deletion of the entire cytochrome P450 CYP2D6 gene as a cause of impaired drug metabolism in poor metabolizers of the debrisoquine/sparteine polymorphism. American Journal of Human Genetics. ,vol. 48, pp. 943- 950 ,(1991)
David B. Duignan, I.Glenn Sipes, Thomas B. Leonard, James R. Halpert, Purification and characterization of the dog hepatic cytochrome P-450 isozyme responsible for the metabolism of 2,2′,4,4′,5,5′-hexachlorobiphenyl Archives of Biochemistry and Biophysics. ,vol. 255, pp. 290- 303 ,(1987) , 10.1016/0003-9861(87)90396-1
Karen M. Kedzie, Scott W. Grimm, Futai Chen, James R. Halpert, Hybrid enzymes for structure-function analysis of cytochrome P-450 2B11. Biochimica et Biophysica Acta. ,vol. 1164, pp. 124- 132 ,(1993) , 10.1016/0167-4838(93)90238-M
Karen M. Kedzie, Richard M. Philpot, James R. Halpert, Functional expression of mammalian cytochromes P450IIB in the yeast Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics. ,vol. 291, pp. 176- 186 ,(1991) , 10.1016/0003-9861(91)90121-X
Hiroko Fukuta, Hiroaki Ohi, Taisuke Uchida, Masayuki Komori, Mitsukazu Kitada, Tetsuya Kamataki, Toxicological sifnificance of dog liver cytochrome P-450: examination with the enzyme expressed in Saccharomycees cerevisiae using recombinant expression plasmid Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. ,vol. 269, pp. 97- 105 ,(1992) , 10.1016/0027-5107(92)90164-W
P. Souček, I. Gut, Cytochromes P-450 in rats: structures, functions, properties and relevant human forms Xenobiotica. ,vol. 22, pp. 83- 103 ,(1992) , 10.3109/00498259209053106
Joyce A. Goldstein, Sonia M. F. de Morais, Biochemistry and molecular biology of the human CYP2C subfamily. Pharmacogenetics. ,vol. 4, pp. 285- 300 ,(1994) , 10.1097/00008571-199412000-00001
Takahiko Katoh, Hisato Inatomi, Akira Nagaoka, Atsuo Sugita, Cytochrome P4501A1 gene polymorphism and homozygous deletion of the glutathione S-transferase M1 gene in urothelial cancer patients. Carcinogenesis. ,vol. 16, pp. 655- 657 ,(1995) , 10.1093/CARCIN/16.3.655