Induction of Osteogenic MC3T3-E1 Cell Differentiation by Nacre and Flesh Lipids of Tunisian Pinctada radiata.
作者： Rym Ben Ammar , Marie‐Hélène Piet , Alice Brion , Khaoula Telahigue , Rim Werheni
DOI: 10.1002/LIPD.12141
关键词: Biochemistry 、 Osteoblast 、 Osteocalcin 、 RUNX2 、 Flesh 、 Fatty acid 、 Radiata 、 Polyunsaturated fatty acid 、 Chemistry 、 Pinctada radiata
摘要: The flesh of the Pinctada radiata pearl oyster from coastal Tunisia is considered as a high source n-3 and n-6 its shell nacre layer promising osteogenic biomaterial. Fatty acid (FA) analysis showed that major components found in total FA (TFA) were 14:0, 16:0, 18:0 saturated (SFA); 16:1, 18:1, 20:1 monoenoic FA; 20:4n-6 (ARA), 22:5n-3 (DPA). Characteristically levels 20:5n-3 (EPA) 22:6n-3 (DHA) (6.53-89.75 mg/100 g TFA) polyunsaturated (PUFA) found, respectively, TFA flesh. Evaluated effects vitro lipids extracted (Ln) (Lc) P. on growth differentiation osteoblasts. Cytotoxicity tests (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide [MTT] lactic dehydrogenase c [LDH]) demonstrated both extracts are nontoxic. Alizarin Red staining was used an osteoblast model using MC3T3-E1 cell line. It induced leading to mineralization. Reverse transcription-polymerase chain reaction (RT-PCR) significantly higher expression osteocalcin (Bglap) runt-related transcription (Runx2) cells presence Ln. No difference osteopontin (Spp1) Collagen type I (Col1a1) genes compared control observed. In conclusion, these results supported, obtained our experimental used, interest/potential stimulate bone formation.
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