In situ hybridization: general infectivity assay for retroviruses.

摘要: We have devised a general infectivity assay for retroviruses. A virus-specific [32P]DNA probe is hybridized in situ to monolayer culture, and foci of infected cells the are detected by exposure culture X-ray films. The method quantitative, that it gives same titer Moloney murine leukemia virus as does standard UV-XC test. specificity indicated fact baboon endogenous do not cross hybridize under conditions used. completed within 1 3 weeks should be broadly applicable retroviruses which replicate without altering cellular morphology: its use demonstrated with mouse mammary tumor helper reticuloendotheliosis complex.