Detection of Viral Nucleic Acids by in Situ Hybridization

摘要: Publisher Summary Methods to adapt hybridization techniques for preparations of cells and chromosomes were devised introduced in 1969 used successfully localize highly reiterated or amplified genes. Improvements both labeling specific probes have extended the compass situ detection single genomes viruses genes chromosomes. These developments herald a new era analysis viral infections role chronic diseases present some exceptional opportunities examine expression individual other disciplines, such as developmental biology. This chapter provides descriptions methods, presents examples use virology, discusses important issues which solution will be greatly influenced by hybridization. In hybridization, nucleic acid probe, labeled radioactively with reporter molecule, is annealed suitably prepared chromosomes, formation hybrids assessed autoradiography, fluorescence, histochemical means. The great increase sensitivity achieved current result increased signal generated contemporary efficiency reflects these improvements, first, preparation and, second, treatment conditions