Regulation of Genes Involved in T-DNA Processing: An Initial Step in the Genetic Modification of Plant Cells

摘要: Directed genetic modification of plants requires the transmission specific foreign DNA to host plant, where is incorporated and stably maintained in plant nuclear genome. Although there are several techniques, transfer mediated through natural T-DNA Ti plasmids Agrobacterium tumefaciens has proven relatively efficient practical. The initial processing genes situated on plasmid bacterial chromosome. located virulence (Vir) region. virD2 gene virD operon, which appears be involved formation intermediates by encoding a endonuclease, constitutively synthesized permissive mutant ros A. tumefaciens. Gene fusions reporter such as luciferase cassette Vibrio fischeri made it possible measure expression Vir during on-going interaction between carrot disks. Using phenolic inducers acetosyringone, virB, virC, virE were identified inducible operons region presence virA, virG inducer. virC also controlled chromosome mutations results constitutive these operons. This enabled us study double stranded absence acetosyringone or other inducers. Integration was directly observed Haplopappus gracilis A situ hybridization analysis.