Mass spectrometry meets the challenge of understanding the complexity of the lipoproteome: recent findings regarding proteins involved in dyslipidemia and cardiovascular disease.

摘要: Despite the fact that link between dyslipidemia and atherosclerosis was made over 100 years ago, remains a major cause of morbidity mortality worldwide. Major efforts focus towards understanding lipid metabolism, particularly by studying its particle compartments in circulation: lipoproteins. In recent years, mass spectrometry has played an integral role deep sequencing lipoproteome metabolism studies conducted vivo. This review highlights path lipoprotein research state-of-the-art with special emphasis on method selected reaction monitoring impact apolipoprotein studies. Also presented is what expected for field advent high resolution/accurate parallel spectrometry. The benefits measurements are demonstrated example instrument workflows detailing novel to quantify very low levels circulating proprotein convertase subtilisin-kexin type 9 rabbit. It anticipated future clinical or trials aimed treat manipulating key regulatory proteins will benefit from new exciting opportunities afforded latest technologies