Side population cells in human gallbladder cancer cell line GBC-SD regulated by TGF-β-induced epithelial-mesenchymal transition
作者： Zhifa Zhang , Feng Zhu , Ling Xiao , Min Wang , Rui Tian
DOI: 10.1007/S11596-011-0671-1
关键词: Carcinogenesis 、 Cell culture 、 Transforming growth factor 、 Cancer research 、 Biology 、 Cancer 、 Abcg2 、 Traditional medicine 、 Side population 、 Epithelial–mesenchymal transition 、 Cancer stem cell
摘要: Mounting evidence has shown that side population (SP) cells are enriched for cancer stem (CSCs) responsible malignancy. In this study, SP technology was used to isolate a small subpopulation of in human gallbladder cell line GBC-SD, and which had superior potential proliferation vitro tumorigenesis vivo were identified. Importantly, the abundance GBC-SD increased by transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT), effect accompanied with strong up-regulation ABCG2 mRNA expression, decreased sensitivity mitoxantrone. restored upon removal TGF-β reversion an epithelial phenotype, smad3-specific siRNA reduced response TGF-β. conclusion, TGF-β-induced EMT smad-dependent signaling pathway promotes development anti-cancer drug resistant phenotype augmenting cells, better understanding mechanisms involved may provide novel strategy preventing progression.
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