A sensitive fluorometric assay for α-chymotrypsin
作者： Elisha Haas , Yehudit Elkana , Richard G. Kulka
DOI: 10.1016/0003-2697(71)90095-9
关键词: Nanotechnology 、 Enzymic hydrolysis 、 Highly sensitive 、 Alpha-chymotrypsin 、 Chromatography 、 Chemistry 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Abstract A rapid, convenient, and highly sensitive fluorometric assay for α-chymotrypsin is suggested. The based on the direct recording of emission intensity β-naphthol liberated by enzymic hydrolysis ZPNE. Microquantities down to 5 × 10 −2 ng are easily assayed. advantage applicability this biological preparations discussed.
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