Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland

摘要: Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related Prorocentrum lima exposure. Oysters were fed with three diets Isochrysis galbana (2 × 10(6) cell mL(-1)) control treatment; algal mix I. galbana (2 × 10(6)) and P. lima (3 × 10(3) cell mL(-1)); alone (3 × 10(3) cell mL(-1)). Feeding behavior changes, histopathological alterations, expression patterns changes genes involved in cell cycle (p21, cafp55, p53), cytoskeleton (tub, act), inflammatory process (casp1) evaluated. Results indicated that presence diarrheic shellfish poisoning by cells decreased clearance rate (p < 0.05), induced structural loss, significantly tubule area digestive gland up-regulated all gene suggesting might trigger tissue process, disturb representing a risk oysters integrity.