Characterization of cell motility in single heart valve interstitial cells in vitro.

摘要: Valve interstitial cells (VIC) are the most prevalent in heart valve, regulating to a large extent normal biology of valve and its pathobiological response disease. In process tissue repair by VICs, single cell motility is likely be important, as it wound mesenchymal type cells. We designed vitro experiments using low density monolayer cultures study association morphology VICs which expressed alpha-smooth muscle actin. observed that can categorized into six types reminiscent shape seen vivo during repair. Of these morphologies, round, rhomboid, tailed spindled shaped were common. did change their over time. Rhomboid could become or spindle-shaped vice versa. Using time-lapse imaging immunofluorescent microscopy, we showed VIC morphologies reflect differences cell-matrix interactions. Tailed predominant motile associated with few extracellular fibronectin fibrils less focal adhesions, demonstrated vinculin staining. Round rhomboid prominent fibrils. found mitosis an important determinant migration. Many daughter separated migrating spindle Thus motility.