A method for measuring bacterial chemotaxis parameters in a microcapillary.
作者： Zewen Liu , Kyriakos D. Papadopoulos
DOI: 10.1002/(SICI)1097-0290(19960705)51:1<120::AID-BIT14>3.0.CO;2-U
关键词: Chemotaxis 、 Measurement method 、 Capillary action 、 Analytical chemistry 、 Waste processing 、 Concentration gradient 、 Diffusion
摘要: A new method was developed which enables chemotaxis parameters to be measured at a single-cell level inside capillary for the first time. The chamber consists of two reservoirs communicating through tube 50 {micro}m in diameter. Chemotaxis are using image analysis, after nearly linear attractant concentration gradient has been generated along by diffusion. Compared previously published techniques, this provides well-characterized chemoattractant profile addition allowing fine capillary. This procedure used measure Escherichia coli K12, and results compared data on single E. cells chemotaxing bulk.
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