Temporal expression profiling of plasma proteins reveals oxidative stress in early stages of Type 1 Diabetes progression

摘要: Abstract Blood markers other than islet autoantibodies are greatly needed to indicate the pancreatic beta cell destruction process as early possible, and more accurately reflect progression of Type 1 Diabetes Mellitus (T1D). To this end, a longitudinal proteomic profiling human plasma using TMT-10plex-based LC-MS/MS analysis was performed track temporal changes T1D patients (n = 11) across 9 serial time points, spanning period natural progression, in comparison with those matching healthy controls (n = 10). our knowledge, current study represents largest (> 2000 proteins measured) expression profiles proteome research. By applying statistical trend on patterns between controls, Benjamini-Hochberg procedure for multiple-testing correction, 13 protein groups were regarded having statistically significant differences during entire follow-up period. Moreover, 16 groups, which play pivotal roles response oxidative stress, have consistently abnormal before seroconversion autoimmunity. Importantly, trends two key reactive oxygen species-decomposing enzymes, Catalase Superoxide dismutase verified independently by ELISA. Biological significance The > 2000 (at least quantified subjects), provided research community. Oxidative stress related different dysregulated group age-sex matched even prior appearance – earliest sign autoimmunity stress.