Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

摘要: The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes evaluate C. gene expression and protein synthesis during infection, using, respectively, an RNAseq-based transcriptomic approach a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes planta were evaluated from 24 84 hours post-inoculation, compared vitro cultures. A total 897 identified interaction with white lupin, which 520 predicted have putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors other species plant 377 hypothetical proteins. Simultaneously, 304 proteins produced the quantified by spectrometry. Taken together, results highlight that dynamics symptoms, shared similarities those hemibiotrophic pathogens. In addition, few unknown poorly-described functions found specifically associated early late stages suggesting they may importance pathogenicity. study, conducted first time on belonging acutatum complex, presents opportunity deepen functional analyses involved spp. onset infection.