Polyvalent Rev Decoys Act as Artificial Rev-Responsive Elements

摘要: Interactions between Rev and the Rev-responsive element (RRE) control order, rate, extent of gene expression in human immunodeficiency virus type 1. decoys may therefore prove to be useful RNA therapeutics for treatment AIDS. To improve upon current generation that bind single molecules, it would generate polyvalent could multiple molecules. J. Kjems P. A. Sharp (J. Virol. 67:4769–4776, 1993) originally constructed functional decoys, but structural context these remains unclear, has been argued individual were either structurally discrete (Kjems Sharp, or part an extended helix (R. W. Zemmel et al., Mol. Biol. 258:763–777, 1996). resolve differences models, we have designed synthesized concatemers Rev-binding elements (RBEs) fold form multiple, discrete, high-affinity sites. We find concatenated RBEs can facilitate cytoplasmic transport viral mRNAs likely These artificial RREs simultaneously sequester hinder access cellular machinery.