Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA

摘要: The Rev trans-activator of human immunodeficiency virus type 1 (HIV-1) is a protein that regulates the simultaneous appearance in cytoplasm both spliced and unspliced forms viral mRNAs from same transcripts by way recognition target sequence termed Rev-responsive element (RRE). Whether acts directly on RNA export or inhibition splicing, both, still matter debate. We have addressed this issue Xenopus laevis oocytes microinjecting molecules containing RRE along with purified recombinant into oocyte nuclei. Adenovirus pre-mRNA an intron was equally well absence presence protein. Only non-spliced exported nucleus; more surprisingly, excised lariat (containing RRE) also exported. Furthermore, RRE-containing mRNA molecule lacked sequences efficiently nucleus Rev-dependent manner. Therefore our results demonstrate can act at level nuclear export, independent any inhibitory effect it may exert splicing pre-mRNA. Finally, finding mutant M10, shown previously to be inactive lymphoid cells, unable nuclei suggests one cellular factors, evolutionarily conserved between humans Xenopus, interact cell systems promote export.