Methanogenesis In Mclean Bog, An Acidic Peat Bog In Upstate New York: Stimulation By H2/CO2 In The Presence Of Rifampicin, Or By Low Concentrations Of Acetate

摘要: Acidic peat bog soils produce CH4 and although molecular biological studies have demonstrated the presence of diverse methanogenic populations in them, few sustained methanogenesis by adding precursors H2/CO2 or acetate, indigenous methanogens been cultured.McLeanBog is a small(ca. 70 m across), acidic (pH 3.4–4.3) Sphagnum-dominated upstate New York. Although addition 10m Macetate stimulated from nearby circumneutral pHfen, neither these substrates led to McLean Bog soil slurries. After brief period stimulation H2/CO2, declined, which could be attributed buildup large amounts acetic acid produced acetogens. Addition antibiotic rifampicin inhibited acetogenesis (carried out Bacteria) allowed Archaea) continue. Using rifampicin, we were able study effects temperature, pH, salts on samples.The enriched H2/CO2-utilizing showed an optimum for activity near pH 5, temperature 35?C.Methanogenesis was not 10 mM acetate,but it 1 multiple additions consumed at increasing rates nearly stoichiometrically converted CH4. In conclusion, found that both hydrogentrophic aceticlastic are present McLeanBog soils, can usingH2/CO2 using low concentrations acetate.