An Integrative Model for Phytochrome B Mediated Photomorphogenesis: From Protein Dynamics to Physiology

摘要: Background Plants have evolved various sophisticated mechanisms to respond and adapt changes of abiotic factors in their natural environment. Light is one the most important environmental it regulates plant growth development throughout entire life cycle. To monitor intensity spectral composition ambient light environment, plants multiple photoreceptors, including red/far-red light-sensing phytochromes. Methodology/Principal Findings We developed an integrative mathematical model that describes how phytochrome B (phyB), essential receptor Arabidopsis thaliana, controls growth. Our based on a multiscale approach connects mesoscopic intracellular phyB protein dynamics macroscopic phenotype. establish reliable relevant parameters for regulated we measured: accumulation degradation, dark reversion kinetics dynamic behavior different nuclear pools using vivo spectroscopy, western blotting Fluorescence Recovery After Photobleaching (FRAP) technique, respectively. Conclusions/Significance The newly predicts phyB-containing bodies (NBs) (i) serve as storage sites (ii) control prolonged well partial reversibility Pfr extended darkness. The predictive power this further validated by fact are able formalize basic photobiological observation, namely light-grown seedlings hypocotyl length depends total amount phyB. In addition, demonstrate our theoretical predictions excellent agreement with quantitative data concerning levels corresponding lengths. Hence, conclude suggested study captures main features phyB-mediated photomorphogenesis Arabidopsis.