A role for Huntington disease protein in dendritic RNA granules
作者： Jeffrey N. Savas , Bin Ma , Katrin Deinhardt , Brady P. Culver , Sophie Restituito
关键词: RNA 、 RNA silencing 、 Gene knockdown 、 Biology 、 Protein biosynthesis 、 P-bodies 、 Argonaute 、 Cell biology 、 Molecular biology 、 Huntingtin 、 Gene silencing
摘要: Regulated transport and local translation of mRNA in neurons are critical for modulating synaptic strength, maintaining proper neural circuitry, establishing long term memory. Neuronal RNA granules ribonucleoprotein particles that serve to along microtubules control protein synthesis response activity. Studies suggest neuronal share similar structures functions with somatic P-bodies. We recently reported the Huntington disease huntingtin (Htt) associates Argonaute (Ago) localizes cytoplasmic P-bodies, which as sites storage, degradation, small RNA-mediated gene silencing. Here we report wild-type Htt Ago2 components co-traffics dendrites. was found co-localize containing 3′-untranslated region sequence known dendritically targeted mRNAs. Knockdown caused altered localization mRNA. When tethered a reporter construct, down-regulated expression manner dependent on Ago2, suggesting may function repress mRNAs during granules.
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