The chicken RH map: current state of progress and microchromosome mapping
作者： M. Morisson , M. Denis , D. Milan , C. Klopp , S. Leroux
DOI: 10.1159/000103160
关键词: Computational biology 、 Microchromosome 、 Expressed sequence tag 、 Reference genome 、 Sequence assembly 、 Genome 、 Genetics 、 Whole genome sequencing 、 Biology 、 Genome project 、 Contig
摘要: The ChickRH6 radiation hybrid panel has been used to construct consensus chromosome (RH) maps of the chicken genome. Markers genotyped were either from throughout genome or targeted specific chromosomes and a large proportion (one third) data was result collaborative efforts. Altogether, 2,531 markers genotyped, allowing construction RH reference for 20 linkage groups four other chromosomes. Amongst markers, 581 belong framework maps, while 1,721 are on comprehensive maps. Around 800 still have be assigned groups. Our attempt assign supercontigs chrun (virtual containing all sequence that could not attributed chromosome) as well EST (Expressed Sequence Tag) contigs do BLAST hit in assembly led new microchromosomes absent which very little is present assembly. presented through our ChickRH webserver (http://chickrh.toulouse.inra.fr/), mapping tool official repository database genotypes. It also displays comparison charts with thus highlighting possible discrepancies. Future improvements include complete coverage chromosomes, further This will help finish smallest gene-rich microchromosomes.
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