In vitro Magnetic Stimulation: A Simple Stimulation Device to Deliver Defined Low Intensity Electromagnetic Fields.

摘要: Non-invasive brain stimulation (NIBS) by electromagnetic fields appears to benefit human neurological and psychiatric conditions, although the optimal parameters underlying mechanisms remain unclear. Although, in vitro studies have begun elucidate cellular mechanisms, is delivered a range of coils (from commercially available laboratory-built circuits) so that induced within tissue produce reported effects are ill-defined. Here, we develop simple device with plug-and-play features allow delivery parameters. We chose test low intensity repetitive magnetic (LI-rMS) at three frequencies hindbrain explant cultures containing olivocerebellar pathway. used computational modeling define circuit coil deliver unidirectional homogeneous field known direction, therefore predictable electric field, target. built be compatible culture requirements: an incubator; flat surface allowing consistent position direction; location outside plate maintain sterility no heating or vibration. Measurements confirmed was homogenous matched simulation results. To validate our system investigated biological following LI-rMS 1 Hz, 10 Hz biomimetic high frequency, which previously shown induces neural reorganization. found gene expression modified frequency-related manner. Four hours after single 10-min session, number c-fos positive cells increased, indicating activated tissue. Also, 14 days LI-rMS, genes normally present differentially according delivered. Thus describe delivers defined different systems vitro. Such devices essential further understanding fundamental on optimize therapeutic application NIBS.