Multiple Particle Tracking for Live Cell Imaging with Green Fluorescent Protein (GFP) Tagged Videos
作者： Sameer Singh , Harish Bhaskar , Jeremy Tavare , Gavin Welsh
DOI: 10.1007/11552499_87
关键词: Pattern recognition (psychology) 、 Particle 、 Live cell imaging 、 Microscope 、 Image segmentation 、 Computer vision 、 Image processing 、 Computer science 、 Adaptive algorithm 、 Artificial intelligence 、 Tracking (particle physics)
摘要: Particle tracking is important for understanding the mobile behaviour of objects varying sizes in a range physical and biological science applications. In this paper we present new algorithm cellular particles imaged using confocal microscope. The performs adaptive image segmentation to identify uses intelligent estimates neighbourhood search, spatial relationship, velocity, direction estimates, shape/size perform robust tracking. Our tracker tested on three videos vesicle GFP tagged videos. results are compared popular Harvard show that our scheme offers better performance flexibility
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Zia Khan, Tucker Balch, Frank Dellaert, An MCMC-Based Particle Filter for Tracking Multiple Interacting Targets european conference on computer vision. pp. 279- 290 ,(2004) , 10.1007/978-3-540-24673-2_23
Howard C. Berg, Motile Behavior of Bacteria Physics Today. ,vol. 53, pp. 24- 29 ,(2000) , 10.1063/1.882934
Laura M. FLETCHER, Gavin I. WELSH, Paru B. OATEY, Jeremy M. TAVARÉ, Role for the microtubule cytoskeleton in GLUT4 vesicle trafficking and in the regulation of insulin-stimulated glucose uptake. Biochemical Journal. ,vol. 352, pp. 267- 276 ,(2000) , 10.1042/BJ3520267
Y. Yasuda, M. Dubois, T.S. Huang, Data compression for check processing machines Proceedings of the IEEE. ,vol. 68, pp. 874- 885 ,(1980) , 10.1109/PROC.1980.11753
Yiider Tseng, Denis Wirtz, Mechanics and Multiple-Particle Tracking Microheterogeneity of α-Actinin-Cross-Linked Actin Filament Networks Biophysical Journal. ,vol. 81, pp. 1643- 1656 ,(2001) , 10.1016/S0006-3495(01)75818-3
G.V. Soni, B.M. Jaffar Ali, Y. Hatwalne, G.V. Shivashankar, Single particle tracking of correlated bacterial dynamics Biophysical Journal. ,vol. 84, pp. 2634- 2637 ,(2003) , 10.1016/S0006-3495(03)75068-1
Hong Qian, Single-Particle Tracking: Brownian Dynamics of Viscoelastic Materials Biophysical Journal. ,vol. 79, pp. 137- 143 ,(2000) , 10.1016/S0006-3495(00)76278-3
Marija Vrljic, Stefanie Y. Nishimura, Sophie Brasselet, W.E. Moerner, Harden M. McConnell, Translational Diffusion of Individual Class II MHC Membrane Proteinsin Cells Biophysical Journal. ,vol. 83, pp. 2681- 2692 ,(2002) , 10.1016/S0006-3495(02)75277-6
Michael K. Cheezum, William F. Walker, William H. Guilford, Quantitative Comparison of Algorithms for Tracking Single Fluorescent Particles Biophysical Journal. ,vol. 81, pp. 2378- 2388 ,(2001) , 10.1016/S0006-3495(01)75884-5
Roger Y. Tsien, THE GREEN FLUORESCENT PROTEIN Annual Review of Biochemistry. ,vol. 67, pp. 509- 544 ,(1998) , 10.1146/ANNUREV.BIOCHEM.67.1.509