The Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT/HIF-1β) is influenced by hypoxia and hypoxia-mimetics

摘要: Background: The Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT, HIF-1β) is a member of the basic-Helix-Loop-Helix PER/ARNT/SIM (bHLH/PAS) protein family and vital transcriptional regulator regarding development physiological adaptation processes. ARNT discussed to be linked with cancer, other diseases. known translocated into cell nucleus, where accumulation takes place. heterodimerisation partner xenobiotic ligand activated (AhR), Single Minded proteins (SIM), cardiovascular helix-loop-helix factor 1 Hypoxia Inducible Factor (HIF-α). obligatory for HIF-1, HIF-2 HIF-3 binding DNA. Whereas degradation HIF-α subunits suppressed by hypoxia, generally regarded as constitutively expressed in excess within cell, stabilisation commonly thought oxygen-independent. However, we provide evidence that regulation far more complex. aim our study was reevaluate expression. Methods: We examined lines different origin like MCF-7 T47D (human breast cancer), HeLa cervix carcinoma), Hep3B HepG2 hepatoma), Kelly neuroblastoma), REPC kidney) Cos7 (primary primate cells. used immunoblot analysis, densitometry, RT-PCR transient transfection. Results Conclusion: Our results show levels are influenced hypoxia mimetics such cobalt(II)-chloride (CoCl2) dimethyloxalylglycine (DMOG) line specific manner. demonstrate this effect might triggered HIF-1α which plays an important role process stabilizing hypoxia.