Attenuation of oxidative stress and chromosomal aberrations in cultured macrophages and pulmonary cells following self-sustained high temperature synthesis of asbestos

摘要: Inhalation of asbestos fibres can cause lung and pleural diseases in humans constitutes a severe public health threat worldwide. The aim the present study was to assess biological effects induced both pulmonary cells (A549) monocyte/macrophage (RAW 264.7) cell lines by combustion slags obtained from through self-sustained high-temperature synthesis (SHS) reaction. SHS reaction involves rapid thermal treatment displays great ability neutralise asbestos. Cytotoxicity, redox status imbalance, lipid peroxide production, DNA strand breaks (comet assay) chromosomal aberrations (cytokinesis block micronucleus test) were evaluated exposed either untreated or grinded SHS-generated different granulometry, tested cultured at varying doses for exposure times. Our results show that especially lines. Moreover, they promote peroxidation trigger genomic alterations. When slag powders, which are products treatment, generation peroxides induction still persisted, due high content iron other metals detected these samples. However, there an attenuation imbalance absence aberrations, probably reflects loss fibrous structure following reaction, as demonstrated electron microscopy analyses. In conclusions, SHS-treated wastes potentially have deleterious oxidative stress inhaled powders but loose induce