作者: A R Tall , E Abreu , J Shuman
DOI: 10.1016/S0021-9258(18)32904-1
关键词: Vesicle 、 Fraction (chemistry) 、 Phospholipid 、 Specific activity 、 Cholesterol 、 Ultracentrifuge 、 Phospholipid transfer protein 、 Biochemistry 、 Adsorption 、 Chromatography 、 Chemistry
摘要: Summary of Purification Procedures-The purification procedure the phospholipid transfer activity is summarized in Table I. Although specific activities are given for each step, only d > 1.19 fraction and material purified through DEAE step were characterized sufficient detail to provide estimates PC under conditions linear dose-response time course. Comparing initial rates (Fig. 1 9), was about 1000-fold at step. The protein further by adsorption vesicles 23 pg PC/3 h/pg protein, indicating approxi- mately 9600-fold purification, relative 1.21 fraction. I shows approximate recoveries expressed as a percentage that preceding Relatively good obtained final steps binding vesicles. In Sepha- rose ultracentrifugation steps, recovery 50 70%, respectively, vesicles, strong CE exchange 6,