Separation of a plasma phospholipid transfer protein from cholesterol ester/phospholipid exchange protein.

作者: A R Tall , E Abreu , J Shuman

DOI: 10.1016/S0021-9258(18)32904-1

关键词: VesicleFraction (chemistry)PhospholipidSpecific activityCholesterolUltracentrifugePhospholipid transfer proteinBiochemistryAdsorptionChromatographyChemistry

摘要: Summary of Purification Procedures-The purification procedure the phospholipid transfer activity is summarized in Table I. Although specific activities are given for each step, only d > 1.19 fraction and material purified through DEAE step were characterized sufficient detail to provide estimates PC under conditions linear dose-response time course. Comparing initial rates (Fig. 1 9), was about 1000-fold at step. The protein further by adsorption vesicles 23 pg PC/3 h/pg protein, indicating approxi- mately 9600-fold purification, relative 1.21 fraction. I shows approximate recoveries expressed as a percentage that preceding Relatively good obtained final steps binding vesicles. In Sepha- rose ultracentrifugation steps, recovery 50 70%, respectively, vesicles, strong CE exchange 6,

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