Partial purification and characterization of a triacylglycerol-transfer protein from rabbit serum
作者： O.V. Rajaram , G.H. White , P.J. Barter
DOI: 10.1016/0005-2760(80)90004-1
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摘要: Abstract Rabbit lipoprotein-free serum was found to contain a protein fraction that mediates the transfer of [ 3 H]triacylglycerol from low-density lipoprotein high-density lipoprotein. Fractionation rabbit by DEAE-Sephadex chromatography, ammonium sulphate precipitation, concanavalin-A-Sepharose Sephadex G-200 gel filtration, phenyl-Sepharose chromatography and G-100 yielded preparation had 500-fold increase in activity compared starting sample. The appeared reside glycoprotein molecular weight range 100000–115000 an isoelectric point at pH 9.
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