A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of cystatin C and β2-microglobulin in urine.
作者： Y Liu , YH Liu , WJ Bei , K Wang , TT Cui
DOI: 10.1080/09674845.2017.1334740
关键词: Urine 、 Chemistry 、 Competitive immunoassay 、 Renal function 、 Contrast-induced nephropathy 、 Fluorescence immunoassay 、 Beta-2 microglobulin 、 Cystatin C 、 Time resolved fluorescence immunoassay 、 Chromatography
摘要: AbstractBackground: Contrast media is widely used in clinical diagnostic and interventional procedures, but may cause damage to the kidney, that is, contrast-induced nephropathy. This study was establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for simultaneous determination of renal function markers cystatin-C (Cys-C) β2-microglobulin (β2-MG) early diagnosis follow-up surveillance nephropathy.Methods: A sandwich immunoassay was detect concentration Cys-C, competitive β2-MG 50 samples urine. The performance this TRFIA evaluated compared with commercial assays.Results: sensitivity Cys-C detection 1.26 ng/ml, average recovery 99.36%; 2.13 ng/ml, 100.18%. Bland–Altman analysis showed method kits had good agreement, s...
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