Inorganic pyrophosphatase-labelled enzyme immunoassay for β2-microglobulin
作者： Heikki Peuravuori , Reijo Kunelius , Jarkko Eskola , Timo Korpela
DOI: 10.1016/S0022-1759(97)00042-2
关键词:
摘要: Abstract β2-Microglobulin was purified from human peritoneal dialysate by ultrafiltration, gel chromatography and DEAE-high performance chromatography. Anti-β2-monoclonal antibodies were developed in mice a pair of the utilized to develop an enzyme-linked immunosorbent assay (ELISA) kit for analyte with utilizing new enzyme label, inorganic pyrophosphatase (EC 3.6.1.1). The sensitivity 0.6 μg/l (3×SD) linear up absorbance values around 2.0. No hook effect occurred any putative concentrations β2-microglobulin serum. precision one run varied within 5–7% CV interassay 2.8–8.6% CV, while recovery 99.2±6.0%. Excellent correlation against established radioimmunoassay method. All used reagents storable minimum 1 year at +4°C. It decided that is label choice ELISA.
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