Inorganic pyrophosphatase as a label in heterogeneous enzyme immunoassay.
作者： A.A. Baykov , V.N. Kasho , S.M. Avaeva
DOI: 10.1016/0003-2697(88)90485-X
关键词: Chromatography 、 Affinity label 、 Phosphatase 、 Chemistry 、 Pyrophosphatase 、 Inorganic pyrophosphatase 、 Enzyme assay 、 Biochemistry 、 Substrate (chemistry) 、 Gel permeation chromatography 、 Color reaction
摘要: Inorganic pyrophosphatase from Escherichia coli has been employed as a label in heterogeneous enzyme immunoassays. Enzyme-antibody conjugates were prepared with the use of glutaraldehyde and purified by gel permeation chromatography. Enzyme activity was measured means sensitive one-step color reaction between phosphate, molybdate, malachite green. The sensitivity terms absorbance readings four to eight times higher than that peroxidase-based assays. change (yellow greenish blue) inherent labeling agent is highly suitable for visual analysis. Other merits include remarkable stability its substrate, compatibility bacteriostatic agents, low Michaelis constant. Examples phosphatase assay human alpha-fetoprotein immunoglobulin G are presented.
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