Peptide nucleic acids as tools for single-molecule sequence detection and manipulation.

作者： Hagar Zohar , Craig L. Hetherington , Carlos Bustamante , Susan J. Muller

关键词: Molecular Probe Techniques 、 Optical tweezers 、 Peptide nucleic acid 、 Molecular probe 、 Fluorophore 、 DNA 、 Biochemistry 、 Peptide 、 Nucleic acid 、 Biophysics 、 Chemistry

摘要: The ability to strongly and sequence-specifically attach modifications such as fluorophores haptens individual double-stranded (ds) DNA molecules is critical a variety of single-molecule experiments. We propose using modified peptide nucleic acids (PNAs) for this purpose implement them in two model experiments where are manipulated via microfluidic flow optical tweezers, respectively. demonstrate that PNAs versatile robust sequence-specific tethers.