The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure
作者： Tzvetelina Tzvetkova-Chevolleau , Angélique Stéphanou , David Fuard , Jacques Ohayon , Patrick Schiavone
DOI: 10.1016/J.BIOMATERIALS.2007.12.016
关键词: Micropatterning 、 Cell adhesion 、 Biophysics 、 Motility 、 Mechanotaxis 、 Cancer cell 、 Extracellular matrix 、 Materials science 、 Extracellular 、 Rigidity (electromagnetism) 、 Nanotechnology
摘要: Cell adhesion and migration are strongly influenced by extracellular matrix (ECM) architecture rigidity, but little is known about the concomitant influence of such environmental signals to cell responses, especially when considering cells similar origin morphology, exhibiting a normal or cancerous phenotype. Using micropatterned polydimethylsiloxane substrates (PDMS) with tunable stiffness (500 kPa, 750 2000 kPa) topography (lines, pillars unpatterned), we systematically analyse differential response (3T3) cancer (SaI/N) fibroblastic cells. Our results demonstrate that both exhibit morphology motility responses changes in substrate rigidity microtopography. 3T3 polarisation spreading microtopography rigidity. The persistent type migration, which depends on anisotropy. In contrast, dynamic SaI/N modified not seem escape from cues: uncorrelated trajectories large dispersion their speed, increases
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