Biosynthesis of purple membrane: control of retinal synthesis by bacterio-opsin

作者: Manfred Sumper , Gisela Herrmann

DOI: 10.1016/0014-5793(76)80964-7

关键词: Laboratory flaskAerationRetinalBiosynthesisChemistryErlenmeyer flaskOpsinSalt solutionMembraneBiochemistryChromatographyBiophysicsGeneticsCell biologyMolecular biologyStructural biology

摘要: as follows: 500 ml 230 medium 100 reciprocating movements per min for 5 days at Low purple membrane production is observed under conditions of sufficient aeration: cells were grown in Erlenmeyer flasks containing only 90 otherwise the presence mM nicotine yielded with medium) or (90 bacteria-opsin, respectively (‘nicotine [7 ] Basal salt solution contained liter 250 g NaCI, 20 MgSO‘, . 7Ha 0 and 2 KCl.

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