作者: Jan Kjellander , Ebba Lund
DOI: 10.1002/J.1551-8833.1965.TB01477.X
关键词: Microbiology 、 Dilution 、 Chromatography 、 Chlorine 、 Chemistry 、 Bacteria 、 Chloramine 、 Escherichia coli 、 Sodium thiosulfate 、 Suspension (chemistry) 、 Agar 、 General chemistry 、 Water Science and Technology
摘要: an agar slant culture of Esch. coli and incubated at 37° C for 4 hr. The broth was then transferred to a bottle containing 100 ml fresh broth, which 18 spun down, the cells were washed twice in phosphate buffer (pH 7) resuspended same buffer. final suspension contained about 109 bacteria per ml. In chlorination experiments, diluted mixed with dilution combined chlorine. At predetermined time intervals samples withdrawn from mixture immediately added solutions sodium thiosulfate con-