The Expression Patterns of BECN1, LAMP2, and PINK1 Genes in Colorectal Cancer Are Potentially Regulated by Micrornas and CpG Islands: An In Silico Study.
作者： Martyna Bednarczyk , Edyta Fatyga , Sylwia Dzięgielewska-Gęsiak , Dariusz Waniczek , Beniamin Grabarek
DOI: 10.3390/JCM9124020
关键词: Gene 、 Epigenetics 、 CpG site 、 In silico 、 Medicine 、 microRNA 、 Tumor promotion 、 Colorectal cancer 、 Gene expression 、 Cancer research
摘要: Background: Autophagy plays a dual role of tumor suppression and promotion in colorectal cancer. The study aimed to find those microRNAs (miRNAs) important BECN1, LAMP2, PINK1 regulation determine the possible epigenetic changes examined cancer using an silico approach. Methods: A total 44 pairs surgically removed tumors at clinical stages I‒IV healthy samples (marginal tissues) from patients’ guts were analyzed. Analysis obtained results was conducted PL-Grid Infrastructure Statistica 12.0 program. miRNAs CpG islands estimated microrna.org database MethPrimer Results: autophagy-related genes shown be able regulated by (BECN1—49 mRNA, LAMP2—62 PINK1—6 mRNA). It observed that regions containing least one region present sequence each gene. Conclusions: analysis performed allowed us mechanisms gene expression, which may interesting therapeutic target treatment
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